Research report 2021 - Max Planck Institute of Biochemistry
Measuring the tRNA world by mim-tRNAseq
Authors
Behrens, Andrew; Rodschinka, Geraldine; Nedialkova, Danny
Departments
Forschungsgruppe Mechanismen der Proteinbiogenese
Summary
Transfer RNAs (tRNAs) specifically deliver amino acids to ribosomes during the translation of messenger RNA (mRNA) into proteins. The abundance of tRNAs therefore has a profound impact on cells. However, determining the amount of each tRNA species in cells was limited because of technical challenges. We overcame these limitations thanks to mim-tRNAseq, a method that can be used to quantify tRNAs in any organism and will help improve our understanding of tRNA regulation in health and disease.